Resources

P30 Grant Collaboration Site (Alfresco) Tutorial Part 1-Uploading Your Files

1. If you're not on the UTHSCSA network, connect to the VPN. Instructions for connecting to the VPN can be found here: http://www.uthscsa.edu/tn/vpn/index.html

2. Load the address in your browser window. The link is http://129.111.167.125:8080/alfresco Remember this will only work on the UTHSCSA network, or via VPN.

3. Change your password. Click the user icon in the upper right hand corner (see fig. 1). This will take you to the user control panel. From there, click the change your password button (fig 2).


Fig 1. Initial View


Fig 2. User control panel
4.Navigating to the site

Once you login, click on the link marked "Company Home" on the upper left corner of the site (fig 3). Next, click "Projects." On the Projects page, click "P30 Grant" which is located in the middle of the page. Finally, click the "Document Library" link in the middle of the page.


Fig 3. Initial login view.

5.Adding Content

Now that you have navigated to the Document Library (as described in step 4) , scroll to the bottom of the page.

The top of the Document Library page (fig 4) shows all documents that have been uploaded or changed, but we want to see the bottom, which displays the folders (called "Spaces" in Alfresco jargon) where you will upload your work.


Fig 4. Document Library (top of page).

Figure 1

Figure 1

figure1.png


Antigen Information for Mouse Mono-/Poly- clonal Antibody

3 mice/antibody x 100 µg antigen/mouse = 300 µg antigen/boost

4 -5 boosts x 300 µg antigen/boost = 1.2 – 1.5 mg antigen

This is the minimum amount of antigen needed for injection.

The cleaner the antigen, the better the response.

For mice, antigen concentrations of 2 mg/ml or higher are preferred because antigen gets diluted with Freund’s Adjuvant for the injection*. Maximum injection volume is ~150 µl, but we prefer injecting ~100 µl.
*NOTE: Antigen can also be left bound to beads. If you do not elute antigen,
it is necessary to wash the resin thoroughly with PBS. Antigen bound to
beads must also be as close to 2 mg/ml (or higher) as possible.

If we end up making monoclonal antibodies, we will need at least 1 mg antigen for ELISA screening.

Mouse injections are usually performed every 2 weeks with bleeds ~10 - 12 days post injection.


Antigen Information for Rabbit Polyclonal Antibody

2 rabbits/antibody x 300 - 500 µg antigen/rabbit = 600 µg - 1 mg antigen/boost

5 boosts x 600 µg - 1 mg antigen/boost = 3 – 5 mg antigen

This is the minimum amount of antigen needed for injection.

The cleaner the antigen, the better the response.

For rabbits, antigen concentrations of 2 mg/ml or higher are needed because antigen gets diluted with Freund’s Adjuvant for the injection*. Maximum injection volume is ~500 µl.
*NOTE: Antigen can also be left bound to beads. If you do not elute antigen,
it is necessary to wash the resin thoroughly with PBS. Antigen bound to
beads must also be as close to 2 mg/ml (or higher) as possible.

Rabbit injections are usually performed every 3 weeks with bleeds ~10 - 14 days post injection.

Serum Totals
5 Total Injections
4 Total Bleeds:
3 Production Bleeds (10 – 20 ml serum) + 1 Final Bleed (50 – 80 ml serum).


Microarray

The Microarray Core is located at:

Cancer Therapy and Research Center’s
The Institute for Drug Development
Alice P. McDermott Building Rm. 216C
14960 Omicron Drive
San Antonio, TX 78245-3217

Microarray Supervisor:
Saurabh K. Gupta, Ph.D.
Director of Genomics
Division of Molecular Pathology
sgupta@idd.org


Shared Resources

The Cancer Therapy and Research Center (CTRC) supports eight basic and three clinical research Shared Resources through its Cancer Center Support Grant (P30 CA54174) from the National Cancer Institute. Shared Resources are defined as centralized facilities and services that provide the membership with expertise and technology to enhance research productivity, foster scientific collaborations, and increase organizational and administrative cohesiveness. Services provided by the Shared Resources are available to scientific members and members of the CTRC. Some services may be provided free of charge to CTRC scientific members, while others are provided at a discounted rate.

The basic science Shared Resources are:

Antigen and Antibody Production
Flow Cytometry
Genomics
Macromolecular Structure
Mass Spectometry
Optical Imaging
Pathology
Protein-Protein Interactions

The clinical research Shared Resources are:

Biostatistics and Medical Informatics
Office of Clinical Research
Pharmacology